報告題目：Droplet Microfluidics for High Throughput Single-Cell Analysis with Single-Molecule Sensitivity
報 告 人：楊朝勇 教授（廈門大學）
報告時間：2015年5月9日下午2：30
報告地點：化學樓二樓一號會議室
報告人簡介：
楊朝勇，男，廈門大學特聘教授、博士生導師、固體表面物理化學國家重點實驗室固定成員、廈門大學化學化工學院化學生物學系副系主任。相繼于1998和2001年在廈門大學化學系獲得學士與碩士學位，2006年獲得美國佛羅里達大學博士學位，2006年9月至2007年12月在美國加州大學伯克利分校從事博士后研究。主要研究領域為生物分析化學，在分子探針、微流控芯片、信號放大、單細胞分析、食品安全等方向取得了創新性的成果。 在PNAS、JACS、Angew Chem Int Ed、Anal Chem等學術刊物上發表論文100多篇，H index 31。研究工作被美國Washington Times、The Scientist、英國RSC Chemistry World、歐洲Wiley Chemistry Views、JACS Spotlights、光明日報等媒體與刊物做了專題報道。先后獲得了美國化學會分析化學研究生獎、中國政府國家優秀自費留學生獎、中美化學與化學生物學教授協會杰出教授獎、中國青年分析化學家獎、福建省五四青年獎章、福建省運盛青年科技獎、福建省高校領軍人才等獎項。2010年獲福建省杰出青年科學基金, 2013年獲國家基金委杰出青年基金。承擔973計劃課題1項、國家自然科學基金5項。現任《分析化學》、Analyst、Biomicrofluidics等期刊編、BMC Biochemistry 副主編。
報告簡介：
It is well established that individual cells, even from the same origin, differ from each other in many aspects due to stochastic biological processes and differences in environmental perturbations. Cell heterogeneity has been found to play an important role in many biological processes, including cellular differentiation and immune response, as well as disease development. The heterogeneity of cells in culture and in organisms poses a challenge for many experimental measurements. Traditional ensemble analysis based on averaging a large population of cells, as a result, masks the behavior of minority subpopulations and effectively blinding researchers to possibly interesting differences between cells. Single-cell analysis is an important and emerging field that gives insights into heterogeneity between cells and advanced cellular processes at high resolution, which is important for cancer research, regenerative medicine, immune system research and diagnostics, as well as for the production of therapeutics. Microfluidics has proven to be a leading tool for single cell analysis since device dimensions are on the same scale as those of cells, allowing for precise fluid and cell manipulation at high throughput. In this talk, I will present our recent efforts on developing droplet microfluidic technology for high throughput single cell isolation, manipulation, and analysis at the DNA, RNA and protein level with single-molecule sensitivity.
